According to The Independent, the two victims, one who says she was a former intern for Signore, posted publically on the social media that Signore used his position to prey on women.
Both women decided to speak up after the investigation, which detailed decades-worth of harassment accusations faced by film producer Harvey Weinstein.
In a Twitter post, Emma Bower claimed that, while interning for Signore's website ScreenJunkies, he made inappropriate comments including one about masturbating over photos of her and in front of her - remarks he later tried to make out were a joke.
Another woman, April Dawn, was a fan of the Screen Junkies show, who met Signore at Chicago Comic Con in 2015. Screen Junkies flew her out to Los Angeles to appear on its Movie Fights YouTube series a few months later.
Dawn wrote that Signore tried to sexually assault her on multiple occasions. She claimed that she and two other women went to the company's HR department, but her complaints of alleged attempted sexual assault were not taken seriously.
Defy Media, which owns the Screen Junkies channel Signore works for, issued a statement saying it has been investigating the accusations.
The statement read, "We at Defy Media have no tolerance for harassment or retaliation of any kind. We have been conducting a thorough investigation into allegations made against Andy Signore and are preparing to respond to those allegations and take appropriate action."
The statement continued, "Given the additional statements that were made today, we feel it is necessary to suspend Andy's employment while we continue our investigation. We will take all necessary action once the investigation is fully complete."
In Dawn's Twitter statement, she said Signore threatened that if she spoke out about what had happened he would fire Dawn's boyfriend, Josh Tapia, a Screen Junkies engineer, who goes by the name JTE and has appeared in Screen Junkies videos.
Signore first gained prominence online as the creator of 'Honest Trailers', a series that skewers films ranging from 'Ghostbusters' to 'Blade Runner'.