The 73-year-old actor has been accused by fellow 'Transparent' actor Trace Lysette, who plays a Yoga instructor, Shea, in the series, of making lewd, sexually suggestive and unwelcome remarks to her several times, reports the Hollywood Reporter.
Lysette has appeared as a guest star in multiple episodes of the show's four seasons to date. She has claimed, "Jeffrey made many sexual advances and comments at me, but one time it got physical."
However, Amazon said that the new information would be added to their ongoing investigation.
According to Lysette, during a break in filming of a scene, Tambor made lewd comments and forced himself on her in a "sexually aggressive manner."
The actress is the second on the 'Transparent' payroll to come forward with allegations that Tambor has subjected them to sexual harassment, sexual assault and generally abusive behaviour on the sets of the critically adored series.
The first accuser, Van Barnes, is a trans woman, who worked as Tambor's personal assistant, and whose allegations have led to an internal investigation by Amazon Studios.
The second season of 'Transparent' traces a father's journey to womanhood, Maura Pfefferman, which is played by Jeffrey Tambor, in a role that has won him two Emmys and struggles to find the right inflection for the popular queer catchphrase.
According to an Amazon spokesperson, "this information will be added to our ongoing conversation."
Tambor has, however, denied the claims. (ANI)